AMSBIO has launched two new biotinylated formats of the 10E4 Heparan Sulfate antibody (high and low biotin) along with a starter pack to allow scientists to test both formats at a reduced cost.
Heparan Sulfate (HS) is a highly sulfated linear polysaccharide that is attached to a core protein to form HS Proteoglycans on the cell membrane or in the extracellular matrix. It is involved in important biological processes due to it displaying specific interactions with many biologically active proteins. In particular, HS has emerged as a key factor in infection by the SARS-CoV-2 virus, which is the cause of the COVID-19 coronavirus pandemic.
Biotinylated antibodies are often used for the detection of low-abundance proteins. The process of biotin-labeling is also frequently used as a non-radiative labeling method for proteins, and as a protein purification technique.
Biotin-conjugation of the Heparan Sulfate 10E4 antibody confers many advantages, by removing the need for conjugated secondary antibodies to quantify the level of detection/binding in immunological assays.
Jacob van den Born and co-workers have used biotinylated Heparan Sulfate 10E4 antibody in double and triple staining experiments**; giving them the freedom to choose the best fluorophore (conjugated to streptavidin) to visualize HS in combination with other antigens. Employing this strategy reduces expenditure on specific secondary antibodies, which are very expensive.
As the 10E4 antibody is a mouse monoclonal antibody, conjugated secondary antibodies will detect any endogenous mouse immunoglobulins already present in the sample as well as the added monoclonal antibody, leading to the risk of “false-positive” results in experiments with adult mouse samples. Using biotinylated Heparan Sulfate 10E4 antibody and enzyme-linked streptavidin, is a way of avoiding this problem.
For further information on biotinylated Heparan Sulfate (10E4) antibodies please visit https://www.amsbio.com/biotinylated-heparan-sulfate-10e4-antibody/ or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / [email protected].
Founded in 1987, AMS Biotechnology (AMSBIO) is recognized today as a leading transatlantic company contributing to the acceleration of discovery through the provision of cutting-edge life science technology, products and services for research and development in the medical, nutrition, cosmetics and energy industries. AMSBIO has in-depth expertise in extracellular matrices to provide elegant solutions for studying cell motility, migration, invasion and proliferation. This expertise in cell culture and the ECM allows AMSBIO to partner with clients in tailoring cell systems to enhance organoid and spheroid screening outcomes using a variety of 3D culture systems, including organ-on-a-chip microfluidics. For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. Drawing upon a huge and comprehensive biorepository, AMSBIO is widely recognised as a leading provider of high-quality tissue specimens (including custom procurement) from both human and animal tissues. The company provides unique clinical grade products for stem cell and cell therapy applications these include high quality solutions for viral delivery (lentivirus, adenovirus and adeno-associated virus) in addition to GMP cryopreservation technology.
** Talsma, Poppelaars, Dam, Meter-Arkema, Vivès, Gál, Boons, Chopra, Naggi, Seelen, Berger, Daha, Stegeman, van den Born & COMBAT Consortium. (2020). MASP-2 Is a Heparin-Binding Protease; Identification of Blocking Oligosaccharides. Frontiers in Immunology, 11, 732.